Head of the Department: prof. dr hab. Jerzy Długoński

Address: 90-237 Łódź, Banacha Street 12/16

Phone: +48 42 635 44 65

Fax: +48 42 665 58 18



Staff of the Department:

Name and surname




Consultation hours

prof. dr hab. Jerzy Długoński

+48 42635 44 65

A / 389

Th. 13:00-14:00

dr hab. Katarzyna Lisowska

(univ. prof.)

+48 42635 44 68

A / 372


Fr.  10:00-12:00


dr hab. Katarzyna Paraszkiewicz

(univ. prof.)

+48 42 635 41 46

A / 63

We.  9:00-10:00


dr Przemysław Bernat (adjunct)

+48 42 635 44 60

A / 275

Fr.  9:00-11:00


dr Aleksandra Felczak

+48 42 635 41 49

A / 274

Mo.  8:00-9:00

We.  8:00-9:00

dr Anna Jasińska (res.  techn.)

+48 42 635 41 46

A / 63


dr Mariusz Krupiński (adjunct)

+48 42 635 45 00

A / 373

Mo.  10:00-11:00

Fr.  11:00-12:00

dr Sylwia Różalska (adjunct)

+48 42 635 41 48

A / 273

We.  11:00-13:00

dr Mirosława Słaba (adjunct)

+48 42 635 41 48

A /273

We. 14:00-15:00

Fr.  11:00-12:00


dr Rafał Szewczyk (adjunct)

+48 42 635 44 60

A / 275

Fr.  14:00-15:00


mgr Tadeusz Bernat (spec. techn.)

+48 42 635 44 60

A / 275


mgr Natalia Wrońska (assisstant)

+48 42 635 44 65

A / 373

Tu.  10:00-12:00



Ph.D students:

Name and surname



mgr Tomasz Janicki

+48 42 635 45 00

mgr Milena Piątek

+48 42 635 41 49

mgr Adrian Soboń

+48 42 635 41 49

mgr Katarzyna Zawadzka

+48 42 635 41 49


Research topics

The research subject of the Department of Industrial Microbiology and Biotechnology has been connected for many years with microbial transformation and production of steroidal drugs. Since 1994 the field of the Department study has been extended to the research on the application of industrial strains for detoxification and biodegradation of harmful xenobiotics, focusing on microbial metabolism of endocrine disrupting chemicals - EDCs. Currently, the scientific work of the Department has a basic and application character and involves the following main areas:

• Microbial manufacture of steroid hormones (hydrocortisone and testosterone) by cholesterol and fitosterols biodegradation, 11-hydroxylation of corticosteroids and androgens reduction (the reactions which also have a detoxification character).

• Biodegradation of polycyclic aromatic hydrocarbons (PAHs) – anthracene and phenanthrene by strains of the filamentous fungus Cunninghamella elegans with high activity of cortexolone transformation to epihydrocortisone and cortisone.

• Biodegradation of toxic metaloorganic compounds, belonging to EDCs (organotin compounds) originating from different matrices – water and soil by fungal industrial strains of Cunninghamella and Curvularia.

• Biodegradation of pentachlorophenol, p-nonylphenol, and other phenol derivatives with EDCs ability by isolated from contaminated soil fungal strain Mucor racemossimus and actimycetes  Streptomyces sp.

• Lead and zinc accumulation as well as degradation of pesticides with EDCs ability by the filamentous fungus Paecilomyces marqundii  isolated from waste of  nonferrous metal works

• Surface active agents production by  strains of the fungus  Curvularia lunata  used in pharmaceutical industry for hydrocortisone manufacture by means of 11α -hydroxylation of corticosteroids and by Bacillus spp.

• Production of lytic enzymes by the filamentous fungus Trichoderma viride, for the degradation carbohydrates, lipids, proteins (a half-technical scale, with the application of bioreactors).

• Investigation of proteome of filamentous fungi capable of xenobiotic degradation.

• Biodegradation of  N-heterocyclic compounds by microscopic fungal strains.

• Biotransformation of fluoroquinolone antibiotics

• Dendrimers as antimicrobial agents

  • Assessments of bactericidal properties of new-synthesized complexes of silver and copper with organic compounds

The studies of the Department (within the main areas mentioned above) are focused mainly (though not exclusively) on the investigation of mechanisms of EDCs biodegradation including:

- cytochrome(s) P-450 involvement in the initial metabolism of investigated pollutants,

- changes in the composition of cell fatty acids induced by the xenobiotics and relationship between biodegradation activity and content  of particular fatty acids,

- application of microcalorimetry and fission yeast (as a bioindicator) for the estimation of the toxic effect(s) of ECDs on microbial degraders,

- studies on the possibilities of simultaneous removal of heavy metals and organic pollutants and effect(s) of heavy metals on biodegradation processes.

- New-synthesized complexes of Ag and Cu with organic compounds with bactericidal activity



Research Projects

-          Microbiological degradation of xenoestrogens and estrogens in the presence of heavy metals and NaCl. 2011-2014

-          Microbial degradation of the chosen N-heterocyclic compounds with pharmacological activity. 2014-2015

-          Effect of biosurfactants produced by Bacillus spp. on biosynthesis of silver nanoparticles (Ag-NPs) and their properties. 2014-2016



-  Institute of Medical Biology. Mycobacterium Genetics and Physiology PAN, Łódź, Poland

-  Faculty of New Technology and Chemistry, Military University of Technology, Warszawa, Poland

-  Military Institute of Chemistry and Radiometry (WICHiR), Warszawa, Poland

-  Laboratory for Basic Research in Horticulture SGGW, Warszawa, Poland

-  National Food and Nutrition Institute, Warszawa, Poland

-  Department of Biochemistry, University of Silesia, Katowice, Poland

-  Environmental Biotechnology Department of Technical University of Gliwice, Poland

-  AB SCIEX sp. z o.o., Poland

-  Avantor Performance Materials Poland POCH, Gliwice, Poland

-  Department of Chemical Engineering University of La Coruna, Spain

-  Laboratory of Systems and Synthetic Biology, University of Wageningen, Netherlands



-          identification and quantification of xenobiotics, and their decomposition products (such as pesticides, steroids, EDCs, amino acids) in samples with complex matrices, using HPLC-MS and GC-MS

-           confocal microscopy

-           elimination of heavy metals and organic xenobiotics from contaminated environments

-           selective recovery of zinc from metallurgical waste

-           the production of steroid hormones (hydrocortisone and testosterone)

-           preparation of lytic enzymes

-           biosynthesis of surface-active substances  (biosurfactants)


Selected publications

1. Paraszkiewicz K., Bernat P., Naliwajski M., Długoński J. Lipid peroxidation in the fungus Curvularia lunata exposed to nickel. Archives of Microbiology (2010) 192:135–141.

2.Różalska S., Szewczyk R., Długoński J. Biodegradation of 4-n-nonylphenol by the non-ligninolytic filamentous fungus Gliocephalotrichum simplex: A proposal of a metabolic pathway. Journal of Hazardous Materials (2010) 180: 323–331.

3.Darlewski W., Popiel S., Nalepa T., Gromotowicz W., Szewczyk R., Stankiewicz R. Investigation of DBS electro-oxidation reaction in the aqueous-organic solution of LiClO4. Journal of Hazardous Materials (2010)175: 460–467.

4.Słaba M., Długoński J. Efficient Zn2+ and Pb2+ uptake by filamentous fungus Paecilomyces marquandii with engagement of metal hydrocarbonates precipitation. International Biodeterioration and Biodegradation (2011) 65, 954-960

5.Piwoński I., Kądzioła K., Kisielewska A., Soliwoda K., Wolszczak M., Lisowska K., Felczak A., Wrońska N. The effect of the deposition parameters on size, distribution and antimicrobial properties of photoinduced silver nanoparticles on titania coatings, Applied Surface Science (2011) 257, 7076-7082.

6.Filipiak E., Walczak-Jedrzejowska R., Krupinski M., Oszukowska E., Marchlewska K., Dlugoński J., Kula K., Slowikowska-Hilczer J. Di(n-butyl) phthalate has no effect on the rat prepubertal testis despite its estrogenic activity in vitro. Folia Histochemica et Cytobiologica, (2011) 49(4)

7.Krupiński M., Długoński J. Biodegradacja nonylofenoli przez wybrane drobnoustroje. Postępy Mikrobiologii (2011) 50: 313-319

8.Ciepluch K, Katir N, El Kadib A, Felczak A, Zawadzka K, Weber M, Klajnert B, Lisowska K, Caminade AM, Bousmina M, Bryszewska M, Majoral JP. Biological properties of new viologen-phosphorus dendrimers. Mol Pharm. (2012) 9(3):448-57.

9.Stankowska D, Czerwonka G, Rozalska S, Grosicka M, Dziadek J, Kaca W. Influence of quorum sensing signal molecules on biofilm formation in Proteus mirabilis O18. Folia Microbiol (Praha). (2012) 57(1):53-60.

10.Karolczak K, Rozalska S, Wieczorek M, Labieniec-Watala M, Watala C. Poly(amido)amine dendrimers generation 4.0 (PAMAM G4) reduce blood hyperglycaemia and restore impaired blood-brain barrier permeability in streptozotocin diabetes in rats. Int J Pharm. (2012) 436(1-2):508-18.

11.Jasińska A, Różalska S, Bernat P, Paraszkiewicz P, Długoński J. Malachite green decolorization by non-basidiomycete filamentous fungi of Penicillium pinophilum and Myrothecium roridum. International Biodeterioration & Biodegradation (2012) 73: 33-40.

12.Rene ER, Bernat P, Długoński J, Veiga MC, Kennes C. Use of styrene as sole carbon source by the fungus Exophiala oligosperma: Optimization and modeling of biodegradation, pathway elucidation, and cell membrane composition. Appl Biochem Biotechnol. (2012) 168(6):1351-71.

13.Felczak A, Wrońska N, Janaszewska A, Klajnert B, Bryszewska M, Appelhans D, Voit B, Różalska S, Lisowska K. Antimicrobial activity of poly(propylene imine) dendrimers. New J. Chem. (2012) 36: 2215-2222.

14.Kowalski K., Szewczyk R., Druszczyńska M. Mycolic acids – potential biomarkers of opportunistic infections caused by bacteria of the suborder Corynebacterineae. Advances in Hygiene and Experimental Medicine. (2012) 66:461-468.

15.Bernat P, Długoński J. Comparative study of fatty acids composition during cortexolone hydroxylation and tributyltin chloride (TBT) degradation in the filamentous fungus Cunninghamella elegans. International Biodeterioration & Biodegradation. (2012) 74:1-6.

16.Gajewska E., Bernat P., Długoński J., Skłodowska M. Effect of Nickel on Membrane Integrity, Lipid Peroxidation and Fatty Acid Composition in Wheat Seedlings. Journal of Agronomy and Crop Science (2012) 198(4). 286-94.

17.Bernat P., Szewczyk R., Krupiński M., Długoński J.. Butyltins degradation by Cunninghamella elegans and Cochliobolus lunatus co-culture. Journal of  Hazardous Materials; (2013) 246-247:277-282.

18.Bernat P., Gajewska E., Bernat T., Wielanek M. Characterisation of the wheat phospholipid fraction in the presence of nickel and/or selenium. Plant Growth Regulation (2013). DOI 10.1007/s10725-013-9848-x

19.Bernat P., Gajewska E., Szewczyk R., Słaba M., Długoński J. Tributyltin (TBT) induces oxidative stress and modifies lipid profile in the filamentous fungus Cunninghamella elegans. Environmental  Science and Pollution Research International; (2013) DOI 10.1007/s11356-013-2375-5

20.Felczak A., Zawadzka K., Wrońska N., Janaszewska A., Klajnert B., Bryszewska M., Appelhans D., Voit B., Lisowska K. Enhancement of antimicrobial activity by co-administration of poly(propylene imine) dendrimers and nadifloxacin. New Journal of Chemistry; (2013) 37, 4156-4162.

21.Kalinowska-Lis U., Felczak A., Chęcińska L., Lisowska K.,  Ochocki J. Synthesis, characterization and antimicrobial activity of silver(I) complexes of hydroxymethyl derivatives of pyridine and benzimidazole. Journal of Organometallic Chemistry; (2013) 749, 394–399.

22.Słaba M., Gajewska E., Bernat P, Fornalska M., Długoński J. Adaptive alterations in the fatty acids composition under induced oxidative stress in heavy metal-tolerant filamentous fungus Paecilomyces marquandii cultured in ascorbic acid presence. Environmental Science and Pollution Research. (2013) 20: 3423-34

23.Słaba M., Szewczyk R., Piątek A., Długoński J.- Oxidation of alachlor by the filamentous fungus Paecilomyces marquandiiJournal of Hazardous Materials.(2013). 261: 443-450.

24.Gajewska E., Niewiadomska E., Tokarz K., Słaba M., Skłodowska M. Nickel-induced changes in carbon metabolism in wheat shoots. J. Plant Physiol. (2013). 170: 369-377

25.Słaba M., Bernat P., Różalska S., Nykiel J., Długoński J. Comparative study of metal induced phospholipid modifications in the heavy metal tolerant filamentous fungus Paecilomyces marquandii and the implication for membrane integrity. (2013). Accepted. Acta Biochimica Polonica.

26.Krupiński M., Szewczyk R., Długoński J. Detoxification and elimination of xenoestrogen nonylphenol by the filamentous fungus Aspergillus versicolor. Int. Biodet. Biodeg. (2013). 82: 59-66.

27.Szewczyk R., Kowalski K., Janiszewska-Drobińska B., Druszczyńska M. Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids. Diag. Microbiol. Infect. Dis. (2013). 76(3): 298–305.

28. Fol M, Iwan-Barańska L, Stączek P, Krupiński M, Różalska S, Kowalewicz-Kulbat M, Druszczyńska M, Madiraju MV, Kaczmarczyk D, Rudnicka W. Interactions between an M. tuberculosis strain overexpressing mtrA and mononuclear phagocytes. Adv Med Sci. (2013). 258(1):172-83.

29. Jasińska A., Bernat P., Paraszkiewicz K. 2013. Malachite green removal from aqueous solution using the system rapeseed press cake and fungus Myrothecium roridum. Desalination and Water Treatment; (2013).  1-9.

30. Felczak A., Bernat P., Długoński J.Biodegradation of octyltin compounds by Cochliobolus lunatus and influence of xenobiotics on fungal fatty acid composition. Process Biochemistry (2013)  Doi: 10.1016/j.procbio.2013.12.001

31. Rozalska S., Pawłowska J., Wrzosek M., Tkaczuk C., Długoński j. Metarhizium sp. nonylphenol biodegradation. (2013). Przyjęte Acta Biochimica Polonica.



Dissertation topics

Examples of dissertations for bachelor degree :

  • The use of mass spectrometry in clinical diagnostic
  • Microbial degradation of quinoline
  • Fluoroquinolone antibiotics – methods of elimination
  • Production and application of  lipopeptide surfactants of microbial origin
  • Microbial degradation of DDT
  • Characteristics,  production and application of the microbiological origin keratinase
  • Characteristics and application lactase produced by microorganisms
  • Application of confocal microscopy in the studies on filamentous fungi
  • Production of vitamins using microorganisms.

Examples of dissertations for master degree:

  • Microbial degradation of acetamiprid and bisphenol A by bacterial strains
  • Degradation of acetamiprid and bisphenol A by filamentous fungi
  • Influence of tributyltin on lipid profile of microscopic fungi Cunninghamella elegans
  • Elimination of technical nonylphenol by Streptomyces sp. IM P102
  • The study of toxic effects of nonylphenols on the Metarhizium anisopliae IM 2358
  • Screening for bacterial strains capable of ethynylestradiol removal
  • The study of ability of Aspergillus versicolor  IM 2161 strain and selected filamentous fungi to eliminate technical nonylophenol
  • The effect of  Ni, Co, Cr III and Cr VI on the surface structures of the Paecilomyces marquandii cells
  • The effect of Pb, Cd, Cu and Zn on the surface structures of the Paecilomyces marquandii cells
  • Determination of the antibacterial activity of polypropyleneimine dendrimers correlated with norfloxacin
  • Determination of the antibacterial activity of polypropyleneimine dendrimers correlated with ciprofloxacin
  • Determination of antibacterial activity of chosen dendrimers
  • Toxicological evaluation of quinoline and its derivatives
  • Proteome study of selected filamentous fungi engaged in the degradation of xenobiotics
  • Biodegradation of  bisphenol A by filamentous fungi
  • Study of malachite green decolorization by microscopic filamentous fungus Penicillium pinophilum IM 6480
  • Study of the mechanisms of malachite green elimination by microscopic filamentous fungi Penicillium pinophilum IM 6480 and Myrothecium roridum IM 6482
  • Characterization of malachite green elimination by microscopic filamentous fungus Myrothecium roridum IM 6482.
  • Screening and preliminary characterization of Bacillus strains capable of surfactants production
  • Silver nanoparticles manufacturing using the filamentous fungi Metarhizium anisopliae