DEPARTMENT OF GENERAL BIOPHYSICS

The Head of the Department: Prof. Maria Bryszewska

Address: 141/143 Pomorska St., 90-236 Lodz, Building D

Phone: +48 42 635 4474

Fax: +48 42 635 4474

e-mail:

 

Name

Phone

e-mail

Building/Room

Consultations

Prof. Maria Bryszewska
(Head of the Department)

+48 42 635 44 74

D /37

Wed.10:30-12:00

Prof. Teresa Gabryelak

+48 42 635 44 78

D/43

Thr.12:00-14:00

Prof. Barbara Klajnert-Maculewicz

+48 42 635 44 29

D/44

Thr.11:00-12:00

Dr. Katarzyna Miłowska

+48 42 635 43 80

D/42

Tue. 11:00-13:00

 

Dr. Hab. Maksim Ionov

 

+48 42 635 43 80

 

 

D/42

 

Dr. Anna Janaszewska

+48 42 635 44 29

 

D/44

 

Dr. Elżbieta Pędziwiatr-Werbicka

+48 42 635 43 80

 

D/42

Fri. 14:00-15:00

Dr. Małgorzata Marszałek

+48 42 635 41 44

 

D/38

 

Monika Błaszczyk

+48 42 635 41 44

D/38

 

 

Ph.D. students:

Name

Phone

e-mail

Aleksandra Szulc

+48 42 635 41 47

 

Małgorzata Ferenc

+48 42 635 41 47

 

Monika Dąbrzalska

+48 42 635 41 47

 

Mgr Monika Marcinkowska

+48 42 635 41 47

 

Mgr Aleksandra Szwed

+48 42 635 41 47

 

Michał Gorzkiewicz

+48 42 635 41 47

Przemysław Trzepiński

+48 42 635 41 47

 

 

Main topics:

The Department of General Biophysics studies a group of polymers, called dendrimers. Dendrimers are characterized by a globular structure and specific architecture. Therefore, they are promising materials for biomedical applications. The main areas of interest are:

  1. Interactions between different types of dendrimers (PAMAM, PPI, sugar modified PPI, polylysine, viologen, carbosilane, phosphorus) with nucleic acids, proteins, biological and model membranes.
  2. Applications of dendrimers in medicine, esp. as carriers of anticancer drugs, and antiamyloid agents in neurodegenerative disorders.
  3. Studies on different types of dendrimers as delivery systems for antisense oligonucleotides (SREV, ANTI TAR, GEM91) and siRNA (siP24, siGAG1) against HIV.
  4. Characterization of dendriplexes (complexes of dendrimers with peptides, nucleic acids) – their morphology, size, surface charge and stability (depending on time, pH, the presence of proteolytic enzymes and other proteins).
  5. Influence of dendrimers and dendriplexes on hemolysis and their impact on transfection and gene silencing.
  6. Toxicity studies of dendrimers and dendriplexes (cytotoxicity for cell lines and toxicity in vivo).
  7. Checking main cell parameters, and DNA damage upon dendrimers and whether dendrimers cause apoptosis.
  8. Biological properties of mesoporous silica.

 

Projects:

  1. FP7-PEOPLE-2012-IRSES NANOGENE EU-Belarus-Russia, Network in Nanomaterials-Driven Anti-Cancer Gene Therapy, 1.I.2013 – 31.XII.2016, co-ordinator: Prof. Maria Bryszewska.
  2. National Science Centre, HARMONIA, Mechanisms of interactions between dendrimers and proteins, Bilateral cooperation between Poland and Belarus, 2012 – 2015, co-ordinator: Prof. Maria Bryszewska.
  3. VENTURES Foundation for Polish Science, PO IG. In vitro studies of phosphorus dendrimers as photosensitizer carriers in PDT therapy combined with hyperthermia. 2013-2015, co-ordinator: Monika Dąbrzalska, M.Sc.
  4. National Science Centre, HARMONIA,Studying phosphorus dendrimers as carriers of photosensitizers. 2013 - 2016, co-ordinator: Prof. Barbara Klajnert-Maculewicz
  5. National Science Centre, OPUS, Dendrimers as drugs for chronic lymphocytic leukemia, 1.XII.2011 – 30.XI.2014, co-ordinator: Prof. Maria Bryszewska.
  6. Ministry of Science and Higher Education, Iuventus Plus, Dendrimers as nanocarriers of drugs used in anti-HIV gene therapy, 2012 – 2013, co-ordinator: Dr. Elżbieta Pędziwiatr-Werbicka.
  7. Ministry of Science and Higher Education (project obtained together with Polish Academy of Science) Synthesis of triphosphonucleosides conjugated with polypropyleneimine (PPI) dendrimers – their anticancer activity in cell cultures. 2011-2013, co-ordinator: PAN, co-ordinator of the task:  Prof. Maria Bryszewska. 

International collaboration:

  1. Collaboration with Biophysical Unit of Autonomous University in Barcelona, Spain – Mechanisms of protein aggregation in neurodegenerative diseases.
  2. Collaboration with Immunomolecular Biology Laboratory in Gregorio Marañon Hospital in Madrid and Inorganic Chemistry Department of University Alcalá de Henares, Madrid, Spain – Dendrimers as carriers of genetic material.
  3. Collaboration with University in Urbino, Institute of Chemical Sciences, Italy – Biophysical methods in studying interactions between dendrimers and biological systems.
  4. Collaboration with Laboratoire de Chimie de Coordination, CNRS, Toulouse, France – Interactions of phosphorus dendrimers with biomolecules.
  5. Collaboration with University in Athens, Department of Pharmaceutical Technology, Greece – Interactions between dendrimers and model lipid bilayers and studies on dendrimer toxicity.
  6. Collaboration with Leibniz Institute fűr Polymerforschung Dresden e.V., Germany – Biological properties of sugar modified dendrimers
  7. Collaboration with Comenius University in Bratislava, Slovakia – Dendrimers as anti-HIV siRNA carriers – interactions with membranes. 
  8. Collaboration with Institute of Biophysics and Cell Engineering of NASB in Minsk, Belarus – Studies of biological and biomedical properties of dendrimers.

Services/Expertise:

  1. Checking cytotoxicity and genotoxicity in vitro.
  2. Impact of different compounds on cell redox.
  3. Enzyme activity assays, determining apoptosis
  4. Model studies with fluorescent dyes.
  5. Studying interactions of drugs, dendrimers, dendriplexes with cell components (proteins, nucleic acids).
  6. Studying alterations of protein, peptide, and nucleic acids structures by circular dichroism spectroscopy (CD) measurements.
  7. Checking stability of colloids by Doppler electrophoresis method.
  8. Estimating the size of nanoparticles by dynamic light scattering (DLS)

Articles:

  1. Ciepluch K., Ionov M., Majoral J.P., Munoz-Fernandez M.A., Bryszewska M., 2014,  Interaction of phosphorus dendrimers with HIV peptides- Fluorescence studies of nano-complexes formation. J. Lumin. 148, 364-369.
  2. Nowacka O., Shcharbin D., Klajenrt-Maculewicz B., Bryszewska M., 2014, Stabilizing  effect of small concentrations of PAMAM dendrimers at the insulin aggregation. Coll. Surf. B: Biointerfaces, DOI: 10.1016/j.colsurfb.2014.01.056.
  3. Pasternak K., Nowacka O., Wróbel D., Pieszyński I.,  Bryszewska M., Kujawa J., 2014, Influence of MLS laser radiation on erythrocyte membrane fluidity and secondary structure of human serum albumin. Mol. Cell. Biochem., 388, 261-267.
  4. Vacas Cordoba E., Bastida H., Pion M., Hameau A., M. Ionov M., Bryszewska M., Caminade A.M., Majoral J.P., Munoz-Fernandez M.A., 2014, HIV-antigens charged on phosphorus dendrimers as tools for tolerogenic DC-based immunotherapy. Curr. Med. Chem. 21, 1-12.
  5. Ziemba B., Franiak-Pietryga I., Pion M., Appelhans A., Munoz-Fernandez M.A., B. Voit B., Bryszewska M., Klajnert-Maculewicz B., 2014, Toxicity and proapoptotic activity of poly(propylene imine) glycodendrimers in vitro: Considering their contrary potential as biocompatible entity and drug molecule in cancer. Int. J. Pharm. 461, 391-402.
  6. Ciepluch K., Weber M., Katir N., Caminade A.M., El Kadib A., Klajnert B., Majoral J.P., Bryszewska M., 2013, Effect of viologen-phosphorus dendrimers on acetylcholinesterase and butyrylcholinesterase activities. Int. J. Biol. Macromol. 54, 119-124.
  7. Ferenc M., Pedziwiatr-Werbicka E., Nowak K.E., Klajnert B., Majoral J-P., Bryszewska M., 2013, Phosphorus dendrimers as carriers of siRNA – Characterisation of dendriplexes.Molecules, 18, 4451-4466.
  8. Halets I., Shcharbin D., Klajnert B., Bryszewska M., 2013, Contribution of hydrophobicity, DNA and proteins to the cytotoxicity of cationic PAMAM dendrimers. Int. J. Pharmaceut. 454, 1-3.
  9. Janaszewska A., Ciołkowski M., Wrobel D., Petersen J.F., Ficker M., Christensen J.B., Bryszewska M., Klajnert B., 2013, Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines. Nanomed. Nanotechnol. Biol. Med. 9, 461-464.
  10. Lazniewska, J. Milowska K., Zablocka M., Mignani S., Caminade A-M., Majoral J-P., Bryszewska M., Gabryelak T., 2013, Mechanism of cationic phosphorus dendrimer toxicity against murine neural cell lines. Mol. Pharmaceut. 10, 3484-3496.
  11. Szulc A., Appelhans D., Voit B., Bryszewska M., Klajnert B., 2013, Studying complexes between PPI dendrimers and Mant-ATP.J. Fluoresc. 23, 349-356.
  12. Ionov M., D. Wrobel, K. Gardikis, S. Hatziantoniou, C. Demetzos, J-P. Majoral, Klajnert B., M. Bryszewska, 2012, Effect of phosphorus dendrimers on DMPC lipid membranes. Chem. Phys. Lipids, 165, 408-413.
  13. Klajnert B., Wasiak T., Ionov M., Fernandez-Villamarin M., Sousa-Herves A., J. Correa J., Riguera R., Fernandez-Megia E., 2012, Dendrimers reduce toxicity of Ab 1-28 peptide during aggregation and accelerate fibryl formation. Nanomed. Nanotech. Biol. Med. 8, 1372-1378.
  14. Lazniewska J., Milowska K., Gabryelak T., 2012, Dendrimers—revolutionary drugsfor infectious diseases.WIREs Nanomed Nanobiotechnol. 4, 469-491.
  15. Maly J., Pedziwiatr-Werbicka E., Maly M., Semeradtova A., Appelhans D., Danani A., Zaborski M., Klajnert B., Bryszewska M., 2012, Highly organized self-assembled dendriplexes based on poly(propylene imine) glycodendrimer and anti-HIV oligodeoxynucleotides. Curr. Med. Chem. 19, 4708-4719.
  16. Milowska K., Gabryelak T., Bryszewska M., Caminade A.M., Majoral J.P., 2012, Phosphorus-containing dendrimers against a-synuclein fibril formation. International Journal of Biological Macromolecules. 50 1138-1143.
  17. Olchowik E., K. Lotkowski, S. Mavlyanov, N. Abdulajanova, M. Ionov, M. Bryszewska, M. Zamaraeva, 2012, Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds. Cell. Mol. Biol. Lett. 17(3).
  18. Ottaviani M. F., Cangiotti M., Fiorani L., Fattori A., Wasiak T., Appelhans D.,  Klajnert B.,  2012, Kinetics of amyloid and prion fibril formation in the absence and presence of dense shell sugar-decorated dendrimers. Curr. Med. Chem. 19, 5907-592.
  19. Pedziwiatr-Werbicka E., D. Shcharbin, J. Maly, M. Maly, M. Zaborski, B. Gabara, P. Ortega, F.J. de la Mata, R. Gomez, M.A. Munoz-Fernandez, B. Klajnert, M. Bryszewska, 2012, Carbosilane dendrimers are a non-viral delivery system for antisense oligonucleotides: characterization of dendriplexes. J. Biomed. Nanotechnol. 8(1), 57-73.
  20. Shakbazau A., Shcharbin D., Petyovka N., Goncharova N., Seviaryn I., Kosmacheva S., Bryszewska M., Potapnev M., 2012, Non-virally modified human mesenchymal stem cells produce ciliary neurotrophic factor in biodegradable fibrin-based 3D scaffolds. J. Pharm. Sci., 101, 1546-1554.
  21. Buczkowski A., Sekowski S., Grala A., Palecz D., Milowska K., Urbaniak P., Gabryelak T., Piekarski H., Palecz B., 2011 Interaction between PAMAM-NH2 G4 dendrimer and 5-fluorouracil in aqueous solution. International Journal of Pharmaceutics. 408, 266-270.
  22. Ciolkowski M., M. Rozanek, M. Szewczyk, B. Klajnert, M. Bryszewska, 2011, The influence of PAMAM-OH dendrimers on the activity of human erythrocytes ATPases. Biochim. Biophys. Acta 1808, 2741-2743.
  23. Ionov M., D. Wróbel, K. Gardikis, S. Hatziantoniou, C. Demetzos, J-P. Majoral, B. Klajnert, M. Bryszewska, 2011, Interaction of cationic phosphorus dendrimers with lipid membranes. Int. J. Nano. Dim. 1(4), 275-278.
  24.  Milowska K., Malachowska M., Gabryelak T., 2011,PAMAM G4 dendrimers affect the aggregation of a-synuclein. International Journal of Biological Macromolecules. 48 742–746.
  25. Sekowski S., Buczkowski A., Palecz B., Gabryelak T., 2011, Interaction of polyamidoamine (PAMAM) succinamic acid dendrimers generation 4 with human serum albumin. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 81, 706-710.
  26. Ziemba B., A. Janaszewska, K. Ciepluch, M. Krotewicz, W.A. Fogel, D. Appelhans, B. Voit, M. Bryszewska, B. Klajnert, 2011, In vivo toxicity of poly(propyleneimine) dendrimers. J. Biomed Mater. Res. Part A, 99 (2), 261-268.
  27. Wrobel D., Kolanowska K., Gajek A., Gomez-Ramirez R., de la Mata F.J., Pedziwiatr-Werbicka E., Klajnert B., Waczulikova I., Bryszewska M., 2014, Interaction of cationic carbosilane dendrimers and their complexes with siRNA with erythrocytes and red blood cell ghosts. Biochim. Biophys. Acta, 1838, 882-889
  28. Franiak-Pietryga I., Ziółkowska E., Ziemba B., Appelhans D., Voit B., Szewczyk M.,  Góra-Tybor J., Robak T., Klajnert B., Bryszewska M., 2013, The influence of maltotriose-modified poly(propylene imine) dendrimers on the chronic lymphocytic leukemia cells in vitro: dense shell G4 PPI. Mol. Pharmaceut. 10, 2490-2501.
  29. Ionov M., Cieluch K., Klajnert B., Glińska S., Gomez-Ramirez R., de la Mata F. J., Munoz-Fernandez M. A., Bryszewska M., 2013, Complexation of HIV derived peptides with carbosilane dendrimers. Colloid Surface B. 101, 236-242.
  30. Lazniewska J., Janaszewska A., Milowska K., Caminade A-M., Mignani S., Katir N., El Kadib A., Bryszewska M., Majoral J-P., Gabryelak T., Klajnert-Maculewicz B., 2013, Promising low-toxicity of violo gen-phosphorus dendrimers against embryonic mouse hippocampal cells.Molecules 18, 12222-12240.
  31. Lazniewska J., Milowska K., Katir N., El Kadib A., Bryszewska M., Majoral J.P., Gabryelak T., 2013, Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line. Cell. Mol. Biol. Lett. 18, 459-478.
  32. Miłowska K., Grochowina J., Katir N., El Kadib A., Majoral J. P., Bryszewska M.,  Gabryelak T., 2013, Viologen-phosphorus dendrimers inhibit a-synuclein fibrillation. Mol. Pharmaceut. 10, 1131-1137.
  33. Milowska K., Grochowina J., Katir N., El Kadib A., Majoral J-P., Bryszewska M., Gabryelak T., 2013, Interaction between viologen-phosphorus dendrimers and a-synuclein. J. Lumin. 134, 132-137.
  34. Neelov I. M., Janaszewska A., Klajnert B., Bryszewska M., Makova N. Z., Hicks D., Pearson H. A., Vlasov G. P., Ilyash M. Y., Vasilev D. S., Dubrovskaya N. M., Tumanova N. L., Zhuravin I. A., Turner A. J., Nalivaeva N. N.,  2013, Molecular properties of lysine dendrimers and their interactions with Aβ-peptides and neuronal cells. Curr. Med. Chem. 20, 134-143.
  35. Vacas Cordoba E., Pion M., Rasines B., D. Fillipini D., Komber H., Ionov M.,  Bryszewska M., Appelhans D., Munoz-Fernadez MA., 2013, Glycodendrimers as new tools in the search for effective anti-HIV DC-based immunotherapies. Nanomed. Nanotech. Biol. Med. 9, 972-984.

Examples of B.Sc. thesis titles:

  1. Interactions between dendrimers and proteins.
  2. Dendrimers as drugs for infectious diseases
  3. Carriers of drugs and genetic materials.
  4. Characterization of phosphorus dendrimers.
  5. The role of dendrimers in anti-HIV therapy.
  6. Toxic proteins in neurodegenerative disorders.
  7. The role of free radicals in neurodegenerative diseases.

 Examples of M.Sc. thesis titles:

  1. Influence of G2.5 and G3.5 dendrimers on red blood cells.
  2. The role of viologen-phosphorus dendrimers in the process of a-synuclein aggregation
  3. Biological properties of nanomolecules with titanium dioxide.
  4. Interactions between PAMAM dendrimers and bovine insulin.
  5. Biological properties of viologen-carbosilane-phosphorus dendrimers.
  6. Toxicity of modified mesoporous silica (type SBA-15).
  7. Impact of dendrimer GATG [G3]-Mor on aggregation of Aβ 1-40 peptide.

Books for students:

Biofizyka dla biologów, Bryszewska M., Leyko W.

Biofizyka. Wybrane zagadnienia wraz z ćwiczeniami, Jóźwiak Z., Bartosz G.

Druga twarz tlenu. Wolne rodniki w przyrodzie, Bartosz G.

Biofizyka, red. Jaroszyk F.

Fizyka wokół nas, Hewitt P.G.

Fizyczne metody diagnostyki medycznej i terapii, red. Hrynkiewicz A.Z., Rokita E.

Useful links:

http://themedicalbiochemistrypage.org/index.php

http://www.ncbi.nlm.nih.gov/sites/gquery

http://www.rcsb.org/pdb/home/home.do

http://ncl.cancer.gov/working_assay-cascade.asp

http://www.gopubmed.org/web/gopubmed/16?WEB0pr2wlm95gdczIrI1gI00h0010009...

http://colinpurrington.com/tips/academic/posterdesign

http://www.cns.cornell.edu/documents/ScientificPosters.pdf