DEPARTMENT OF CYTOBIOCHEMISTRY


The Head of the Department: Anna Krześlak, Ph. D., Associate professor

 

Address: 141/143 Pomorska St., 90-236 Lodz, Building D

 

Tel: +48 42 635 43 35

Fax: +48 42 635 44 84

e-mail: anna.krzeslak(at)biol.uni.lodz.pl

Adres/link: www.cytobiochemia.uni.lodz.pl/indexeng.html

  

Staff:

Name

Phone

e-mail

Building/

room

Consultations and assistance hours

Anna Krześlak, Ph.D., D.Sc. Associate professor

Head of the Department

635 43 35

anna.krzeslak(at)biol.uni.lodz.pl

D / Bch58

 

Wanda Małgorzata Krajewska, Professor,

635 44 87

wanda.krajewska(at)biol.uni.lodz.pl

D / Bch60

 

Magdalena Bryś, Professor

635 43 71

magdalena.brys(at)biol.uni.lodz.pl

D / Bch61

 

Małgorzata Rogalińska, Ph.D., D.Sc., Associate professor

635 43 79

malgorzata.rogalinska(at)biol.uni.lodz.pl

D / Bch59

 

Adam Cygankiewicz, Ph.D., Adjunct

635 52 99

adam.cygankiewicz(at)biol.uni.lodz.pl

D / Bch10

 

Ewa Forma, Ph.D., Adjunct

635 52 99

ewa.forma(at)biol.uni.lodz.pl

D / Bch13

 

Paweł Jóźwiak, Ph.D., Adjunct

635 44 03

pawel.jozwiak(at)biol.uni.lodz.pl

D / Bch19

 

Piotr Zakrzewski, Ph.D., Adjunct

635 52 99

 

D / Bch10

 

Jolanta Żołnierczyk, Ph.D., Adjunct

 

 

D / Bch16

 -

Adam Kurzac, M.Sc., Technician

635 44 84

 

D / Bch12

 -

Monika Szczepańska, B.Sc., Technician

635 44 84

monika.szepanska(at)biol.uni.lodz.pl

D / Bch12

 -

 

PhD students:

Name

Phone

e-mail

Building/

room

Consultations and assistance hours

Piotr Ciesielski, M.Sc.

635 44 03 

piotr.ciesielski(at)biol.uni.lodz.pl

D / Bch19

 

Damian Jacenik, M.Sc.

 

 

D / Bch10

 -

Oleksandra Liudvytska, M.Sc.

  

D / Bch49

 

Paulina Machała, M.Sc.

 paulina.machala(at)uni.lodz.euD / Bch49 

Aleksandra Szustka, M.Sc.

635 44 89 

aleksandra.szustka(at)biol.uni.lodz.pl

D / Bch16

 

Agnieszka Zaczek, M.Sc.

 635 44 89agnieszka.zaczek(at)biol.uni.lodz.plD / Bch16 Wed 10:00-12:00

 

Research topics:

  1. Identification of potential tumor markers of B-cell chronic lymphocytic leukemia and breast, prostate, endometrial, bladder and colon cancers
  2. Alterations of cellular signal transmission in neoplastic transformation and in inflammatory diseases
  3. Epigeneticmodifications in cancer
  4. Protein glycosylation and regulation of cancer cells metabolism
  5. Personalization of chronic lymphocytic leukemia treatment

 

Researchprojects/grants :

  1. Role of mitochondrial O-GlcNAc transferase isoform in glucose-dependent regulationof glycolytic activity in normal and cancer breast cells; PawełJóźwiak, 2016-2019, National Science Centre,  UMO-2015/19/D/NZ3/01488
  2. Estrogen receptor GPER as a modulator of inflammatory and functional gastrointestinal diseases and colorectal cancer; Damian Jacenik, 2016-2019, National Science Centre, UMO-2015/17/N/NZ5/00336
  3. The role of interplay between TET3 and O-GlcNAc transferase in progression of cancer; Piotr Ciesielski,  2016-2019,National Science Centre  UMO-2015/19/N/NZ3/01311
  4. The impact of O-GlcNAc transferase on Polycomb dependent repression of genes associated with differentiation of breast cancer cells,  Anna Krześlak, 2013-2016,National Science Centre UMO-2012/07/B/NZ3/00234
  5. Molecular basis of bataglycan deregulation in endometrial cancer; Piotr Zakrzewski, 2011-2013, National Science Centre, 2011/01/N/NZ4/01723
  6. Personalized medicine for CLL; MałgorzataRogalińska, 2011-2013,National Science Centre 2011/01/B/NZ4/01702
  7. Impact of protein O-GlcNAc modification on AKT signaling pathway in thyroid cancer; Anna Krześlak,2008-2010; Ministry of Science and Higher Education N N301 463534

 

Cooperation:

  1. II Department of Gynecology, Medical University of  Lublin, Poland
  2. General and Colorectal Surgery Department, Medical University of Lodz, Poland
  3. Clinical Division of Gynecologycal Oncology, Medical University of Lodz, Poland
  4. Department of Gastroenterology, Medical University of Lodz, Poland
  5. Department of Operative Gynaecology, Polish Mother's Memorial Hospital - Research Institute, Lodz, Poland
  6. Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Poland
  7. I Department of Otolaryngology and Laryngological Oncology, Medical University of Lodz, Poland
  8. 2nd Clinic of Urology, Medical University of  Lodz, Poland
  9. Maria Sklodowska-Curie Memorial Hospital,Medical University of Lodz, Zgierz, Poland
  10. Department of Biochemistry, Medical University of Lodz, Poland
  11. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  12. Department of Physical Chemistry, Faculty of Chemistry, University of Lodz, Poland
  13. Department of Environmental Chemistry, Faculty of Chemistry , University of Lodz , Poland
  14. Institute of Medical Biology of PAS, Lodz, Poland
  15. Department of Pathology, Polish Mother Memorial Hospital Research Institute, Lodz, Poland
  16. Department of Biochemistry and Molecular Biology, University of Chicago, IL, USA
  17. Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, USA
  18. Department of Pharmacology, University of Texas Health Science Center, San Antonio, TX, USA

 

Selected publications:

  1. Jacenik D, Cygankiewicz AI, Krajewska WM. The G protein-coupled estrogen receptor as a modulator of neoplastic transformation. Mol Cell Endocrinol. 2016, 429:10-8. doi: 10.1016/j.mce.2016.04.011.
  2. Żołnierczyk JD, Olejniczak AB, Mieczkowski A, Błoński JZ, Kiliańska ZM, Robak T, Leśnikowski ZJ.In vitro antileukemic activity of novel adenosine derivatives bearing boron cluster modification.Bioorg Med Chem. 2016 ,doi: 10.1016/j.bmc.2016.08.028
  3. Rogalinska M. The Role of Mitochondria in Cancer Induction, Progression and Changes in Metabolism. Mini Rev Med Chem. 2016;16(7):524-30
  4. Salaga M, Blomster LV, Piechota-Polańczyk A, Zielińska M, Jacenik D, Cygankiewicz AI, Krajewska WM, Mikkelsen JD, Fichna J. Encenicline, an α7 Nicotinic Acetylcholine Receptor Partial Agonist, Reduces Immune Cell Infiltration in the Colon and Improves Experimental Colitis in Mice.JPharmacolExpTher. 2016,356(1):157-69. doi: 10.1124/jpet.115.228205
  5. Starska K, Forma E, Nowacka-Zawisza M, Lewy-Trenda I, Ciesielski P, Pietruszewska W, Skóra M, Bryś M. The c.*229C > T gene polymorphism in 3'UTR region of the topoisomerase IIβ binding protein 1 gene and LOH in BRCA1/2 regions and their effect on the risk and progression of human laryngeal carcinoma. Tumour Biol. 2016, 37(4):4541-57. doi: 10.1007/s13277-015-4276-3.
  6. Zakrzewski PK, Nowacka-Zawisza M, Semczuk A, Rechberger T, Gałczyński K, Krajewska WM. Significance of TGFBR3 allelic loss in the deregulation of TGFβ signaling in primary human endometrial carcinomas. Oncol Rep. 2016, 35(2):932-8. doi: 10.3892/or.2015.4400.
  7. Nowacka-Zawisza M, Forma E, Walczak M, Różański W, Bryś M, Krajewska WM. Loss of heterozygosity for chromosomal regions 15q14-21.1, 17q21.31, and 13q12.3-13.1 and its relevance for prostate cancer. Med Oncol. 2015, 32(11):246. doi: 10.1007/s12032-015-0691-y.
  8. Jóźwiak P, Krześlak A, Wieczorek M, Lipińska A. Effect of Glucose on GLUT1-Dependent Intracellular Ascorbate Accumulation and Viability of Thyroid Cancer Cells. Nutr Cancer. 2015;67(8):1333-41. doi: 10.1080/01635581.2015.1078823.
  9. Rogalińska M, Błoński JZ, Góralski P, Wawrzyniak E, Hartman M, Rogalska A, Robak P, Koceva-Chyła A, Piekarski H, Robak T, Kiliańska ZM. Relationship between in vitro drug sensitivity and clinical response of patients to treatment in chronic lymphocytic leukemia. International Journal Oncology 2015 , 46(3):1259-67.
  10. Starska K, Forma E, Brzezińska-Błaszczyk E, Lewy-Trenda I, Bryś M, Jóźwiak P, Krześlak A. Gene and protein expression of O-GlcNAc-cyclingenzymes in humanlaryngealcancer. ClinExp Med. 2015, 15(4):455-68. doi: 10.1007/s10238-014-0318-1.
  11. Nowacka-Zawisza M, Wiśnik E, Wasilewski A, Skowrońska M, Forma E, Bryś M, Różański W, Krajewska WM.Polymorphisms of homologous recombination RAD51, RAD51B, XRCC2, and XRCC3 genes and the risk of prostate cancer.Anal Cell Pathol (Amst). 2015; doi: 10.1155/2015/828646.
  12. Rogalinska M, Kilianska Z. M.Personalized therapy versus targeted therapy, differences in the meaning. Global Journal for Research Analysis 4(1):5-8.
  13. Jóźwiak P, Forma E, Bryś M, Krześlak A. O-GlcNAcylation and Metabolic Reprograming in Cancer. Front Endocrinol (Lausanne). 2014,5:145. doi: 10.3389/fendo.2014.00145. eCollection 2014.
  14. Forma E, Jóźwiak P, Bryś M, Krześlak A. The potential role of O-GlcNAc modification in cancer epigenetics. Cell MolBiol Lett. 2014,19(3):438-60. doi: 10.2478/s11658-014-0204-6.
  15. Goralski P, Rogalinska M,  Błonski J. Z, Pytel E, Robak T, Kilianska ZM, Piekarski H The differences in thermal profiles between normal and leukemic cells exposed to anticancer drug evaluated by differential scanning calorimetry, Journal Thermal Analytical Calorimetry (2014) 118:1339–44.
  16. Żołnierczyk JD, Borowiak A, Hikisz P, Cebula-Obrzut B, Błoński JZ, Smolewski P, Robak T, Kiliańska ZM. Promising anti-leukemic activity of atorvastatin.Oncol Rep. 2013 May;29(5):2065-71. doi: 10.3892/or.2013.2301. Epub 2013 Feb 22.
  17. Cygankiewicz AI, Maslowska A, Krajewska WM. Molecular basis of taste sense: involvement of GPCR receptors. CritRev Food SciNutr. 2014; 54(6):771-80. doi: 10.1080/10408398.2011.606929
  18. Rogalińska M, Franiak-Pietryga I, Błoński JZ, Góralski P, Maciejewski H, Janus A, Robak P, Mirowski M, Piekarski H, Robak T, Kiliańska ZM. Toward personalized therapy for chronic lymphocytic leukemia: DSC and cDNA microarray assessment of two cases. CancerBiologyTherapy. 2013 Jan;14(1):6-12.
  19. Forma E, Wójcik-Krowiranda K, Jóźwiak P, Szymczyk A, Bieńkiewicz A, Bryś M, Krześlak A. Topoisomerase IIβ binding protein 1 c.*229C>T (rs115160714) gene polymorphism and endometrial cancer risk. PatholOncol Res. 2014, 20(3):597-602. doi: 10.1007/s12253-013-9737-7.
  20. Fichna J, Wood JT, Papanastasiou M, Vadivel SK, Oprocha P, Sałaga M, Sobczak M, Mokrowiecka A, Cygankiewicz AI, Zakrzewski PK, Małecka-Panas E, Krajewska WM, Kościelniak P, Makriyannis A, Storr MA. Endocannabinoid and cannabinoid-like fatty acid amide levels correlate with pain-related symptoms in patients with IBS-D and IBS-C: a pilot study. PLoS One. 2013 Dec 27;8(12):e85073. doi: 10.1371/journal.pone.0085073. eCollection 2013.
  21. Rogalinska M, Kilianska ZM. Targeting Bcl-2 in CLL.  Current Medicinal Chemistry 2012;19(30):5109-15.

 

Bachelor degree dissertation topics:

  1. Cancer cell biology
  2. Epigentetic regulation of gene expression
  3. Nuclear and mitochondrial genomes
  4. Stem cells
  5. Knowledge of cell biology as a basis of modern therapeutic strategies
  6. Molecular biology in forensic medicine

 

Examples of BSc thesis titles:

  1. Genetic instability in cancer development
  2. Alterations of protein folding in neurodegenerative diseases
  3. Lipid rafts – microdomains of biological membranes – structure and function
  4. Atherosclerosis influence on coronary artery disease

 

Examples of MSc thesis titles:

  1. Expression of SLUG and TWIST1 genes in endometrial cancer
  2. Expression of selected microRNA in colon pathophysiology
  3. RAD51 and RAD51B genes polymorphism in inflammatory bowel diseases
  4. Impact of O-GlcNAc transferase on expression and localization of TET  in breast cancer cells
  5. Evaluation of antileukemic activity of purine derivatives and atorvastatin

 

Services/Expertise/Training:

Conducting the analyses that involve methods used in the Department:

  1. Cell culture
  2. PCR, RFLP-PCR,MSP-PCR
  3. Real-Time PCR
  4. DNA sequencing
  5. Western blot
  6. ELISA
  7. Assessment of proapoptotic activity of drugs used for leukemia therapies
  8. Assessment of impact of potential drugs  and biologically active substances on genes expression levels

 

For students:

Monographic lectures in English:

  1. Anna Krześlak, Ph.D., D.Sc. Associate professor – Cancer epigenetics – summer semester, 13 h
  2. Anna Krześlak, Ph.D., D.Sc. Associate professor  -  Epigenetics and lifestyle – summer semester, 13 h